Validating rnai targets
Using any form of DNA construct, except the PCR template format found in Allele’s Line Silence platform, requires creating DNA constructs and sequence verification; a taxing work load if multiple genes need to be studied.However, once the constructs are made, they can be reproduced easily and inexpensively.H1, U6) that are popularly used as gene silencing tools.Compared to other sh RNA-expressing plasmids, Allele’s Silen Circle platform design allows high-level expression of sh RNA.Allele Biotech was one of the very first companies to offer synthetic si RNA and RNA polymerase III promoter systems for expressing sh RNA.Allele Biotech has been granted multiple patents (US patents 7,294,504, 7,422,896, 7,625,750, and China patent CN02828345.7) on DNA-based si RNA and sh RNA platforms that are popularly used as gene silencing tools.
Another drawback of using synthetic si RNA is the limited duration of post-transfection effects, with gene silencing activities peaking at around 24 hours, and then diminishing within 48 RNA can be introduced via DNA plasmid, linear template, or packaged retroviral/lentiviral vectors.
That has been a reason why researchers resort to validate RNAi target sequences by themselves or through custom services.
With Allele Biotech's powerful and unique viral packaging services, patent-protected RNAi platform, and state-of-the-art fluorescent protein markers, we provide a full service for RNA interference, including RNAi expression viral packaging, fluorescent protein-based RNAi validation, and even large scale RNAi screening.
To solve this problem Ori Gene has created a fusion construct, which incorporates a full-length Ori Gene True Clone and a luciferase reporter gene, the RNAi Validation Vector.
Using this new tool it is possible to measure the effectiveness of RNAi constructs with targeted specificity using nothing more than a luminescence reader.